磷酸二酯酶Ⅱ

英文名稱：Phosphodiesterase II from bovine spleen；3′-Exonuclease；Spleenexonuclease；Spleen phosphodiesterase

其他名稱：磷酸二脂酶；磷酸二酯酶

CAS號：9068-54-6

級別：BR

Specific activity：≥1.2u/mg protein

活力定義：One Unit increases the absorbance at 260 nm by 0.200 in 30minutes at 37℃, pH 6.5, with an RNA substrate

產品描述：Spleen exonuclease (Phosphodiesterase II) excises3'-phosphomononucleotides from oligonucleotides having a free OH terminus. Theoptimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification ofthe Hilmoe (Biochem. Prep., VII, (Meister, A., ed.), John Wiley and Sons, NY,105, 1961) technique

性狀(以下信息僅供參考)：粉末

用途：本品僅供科研，不得用于其它用途

保存：-20℃