PKB Elisa Kit鍘熺悊锛�

ELISA瑭﹀姂鐩掓槸鍥虹浉澶惧績娉曢叾鑱�(li谩n)鍏嶇柅鍚搁檮瀵�(sh铆)椹�(y脿n)锛圗LISA锛�.宸茬煡寰呮脯(c猫)鐗╄唱(zh矛)婵冨害鐨勬(bi膩o)婧�(zh菙n)鍝併€佹湭鐭ユ績搴︾殑妯ｅ搧鍔犲叆寰瓟閰舵(bi膩o)鏉垮収(n猫i)閫�(j矛n)琛屾娓�(c猫)銆傚厛灏囧緟娓�(c猫)鐗╄唱(zh矛)鍜岀敓鐗╃礌妯�(bi膩o)瑷樼殑鎶楅珨鍚屾檪(sh铆)婧偛銆傛礂婊屽悗锛屽姞鍏ヨΚ鍜岀礌妯�(bi膩o)瑷橀亷(gu貌)鐨凥RP銆傚啀缍�(j墨ng)閬�(gu貌)婧偛鍜屾礂婊岋紝鍘婚櫎鏈祼(ji茅)鍚堢殑閰剁祼(ji茅)鍚堢墿锛岀劧鍚庡姞鍏ュ簳鐗〢銆丅锛屽拰閰剁祼(ji茅)鍚堢墿鍚屾檪(sh铆)浣滅敤銆傜敘(ch菐n)鐢熼鑹层€傞鑹茬殑娣辨泛鍜屾ǎ鍝佷腑寰呮脯(c猫)鐗╄唱(zh矛)鐨勬績搴﹀憟姣斾緥闂�(gu膩n)绯汇€�

PKB Elisa Kit 绲勬垚锛�

鑷倷鏉愭枡

1锛夎捀椁炬按銆�

2锛夊姞妯ｅ櫒锛�5ul銆�10ul銆�50ul銆�100ul銆�200ul銆�500ul銆�1000ul銆�

3锛夋尟钑╁櫒鍙婄鍔涙敧鎷屽櫒绛夈€�

PKB Elisa Kit 妯�(bi膩o)鏈姹�

1.妯�(bi膩o)鏈噰闆嗗悗鐩℃棭閫�(j矛n)琛屾彁鍙栵紝鎻愬彇鎸夌浉闂�(gu膩n)鏂囩嵒(xi脿n)閫�(j矛n)琛岋紝鎻愬彇鍚庢噳(y墨ng)鐩″揩閫�(j矛n)琛屽(sh铆)椹�(y脿n)銆傝嫢涓嶈兘棣笂閫�(j矛n)琛岃│椹�(y脿n)锛屽彲灏囨(bi膩o)鏈斁浜�-20鈩冧繚瀛橈紝浣嗘噳(y墨ng)閬垮厤鍙嶅京(f霉)鍑嶈瀺

2.涓嶈兘妾㈡脯(c猫)鍚� NaN3 鐨勬ǎ鍝侊紝鍥� NaN3 鎶戝埗杈ｆ牴閬�(gu貌)姘у寲鐗╅叾鐨勶紙HRP锛夋椿鎬с€�

2.鎿嶄綔姝ラ

1.绶ㄨ櫉(h脿o)锛氬皣妯ｅ搧灏�(du矛)鎳�(y墨ng)寰瓟鎸夊簭绶ㄨ櫉(h脿o)锛屾瘡鏉挎噳(y墨ng)瑷�(sh猫)闄版€у皪(du矛)鐓� 2 瀛斻€侀櫧(y谩ng)鎬у皪(du矛)鐓�2 瀛斻€佺┖鐧藉皪(du矛)鐓� 1

瀛旓紙绌虹櫧灏�(du矛)鐓у瓟涓嶅姞妯ｅ搧鍙婇叾妯�(bi膩o)瑭﹀姂锛屽叾浣欏悇姝ユ搷浣滅浉鍚岋級

2.鍔犳ǎ锛氬垎鍒ュ湪闄般€侀櫧(y谩ng)鎬у皪(du矛)鐓у瓟涓姞鍏ラ櫚鎬у皪(du矛)鐓с€侀櫧(y谩ng)鎬у皪(du矛)鐓� 50渭l銆傜劧鍚庡湪寰呮脯(c猫)妯ｅ搧瀛斿厛鍔犳ǎ鍝佺█閲嬫恫 40渭l锛岀劧鍚庡啀鍔犲緟娓�(c猫)妯ｅ搧 10渭l銆傚姞妯ｅ皣妯ｅ搧鍔犱簬閰舵(bi膩o)鏉垮瓟搴曢儴锛岀洝閲忎笉瑙稿強瀛斿锛岃紩杓曟檭鍕�(d貌ng)娣峰嫽锛�

3.婧偛锛氱敤灏佹澘鑶滃皝鏉垮悗缃� 37鈩冩韩鑲� 30 鍒嗛悩銆�

4.閰嶆恫锛氬皣 30 鍊嶆績绺礂婊屾恫鐢ㄨ捀椁炬按 30 鍊嶇█閲嬪悗鍌欑敤

5.娲楁粚锛氬皬蹇冩彮鎺夊皝鏉胯啘锛屾鍘绘恫楂旓紝鐢╁共锛屾瘡瀛斿姞婊挎礂婊屾恫锛岄潨缃� 30 绉掑悗妫勫幓锛屽姝ら噸寰�(f霉) 5 娆★紝鎷嶅共銆�

6.鍔犻叾锛氭瘡瀛斿姞鍏ラ叾妯�(bi膩o)瑭﹀姂 50渭l锛岀┖鐧藉瓟闄ゅ銆�

7.婧偛锛氭搷浣滃悓 3銆�

8.娲楁粚锛氭搷浣滃悓 5銆�

9.椤壊锛氭瘡瀛斿厛鍔犲叆椤壊鍔� A50渭l锛屽啀鍔犲叆椤壊鍔� B50渭l锛岃紩杓曢渿钑╂贩鍕伙紝37鈩冮伩鍏夐’鑹�10 鍒嗛悩.

10.绲傛锛氭瘡瀛斿姞绲傛娑� 50渭l锛岀祩姝㈠弽鎳�(y墨ng)锛堟鏅�(sh铆)钘�(l谩n)鑹茬珛杞�(zhu菐n)榛冭壊锛夈€�

娓�(c猫)瀹氾細浠ョ┖鐧界┖瑾�(di脿o)闆讹紝450nm 娉㈤暦(zh菐ng)渚濆簭娓�(c猫)閲忓悇瀛旂殑鍚稿厜搴︼紙OD 鍊硷級銆� 娓�(c猫)瀹氭噳(y墨ng)鍦ㄥ姞绲傛娑插悗 15 鍒嗛悩浠ュ収(n猫i)閫�(j矛n)琛屻€�

PKB Elisa Kit 鏁�(sh霉)鎿�(j霉)瑷�(j矛)绠�

1. 绻埗妯�(bi膩o)婧�(zh菙n)鏇茬窔锛氬悇妯�(bi膩o)婧�(zh菙n)鍝� OD 鍊兼笡鍘诲簳鑹插嵆娓涘幓绌虹櫧瀛� OD 鍊硷紝浠� 6 鍊�(g猫)妯�(bi膩o)婧�(zh菙n)

鍝佺殑 OD 鍊肩偤绺卞潗妯�(bi膩o) y锛屼互妯�(bi膩o)婧�(zh菙n)鍝佺殑婵冨害鐐烘┇鍧愭(bi膩o) x锛岀躬鍒舵洸绶氬湒锛屽鍦栫ず锛�

骞惰▓(j矛)绠楀嚭鍥炴鏂圭▼ y=ax+b銆�

2. 灏囧緟娓�(c猫)妯ｅ搧鐨� OD 鍊间唬鍏ユ柟绋嬪紡锛岃▓(j矛)绠楀悇绲勬ǎ鍝佹績搴︼紝鍐嶄箻浠ョ█閲嬪€嶆暩(sh霉)锛屽嵆

鐐烘ǎ鍝佺殑瀵�(sh铆)闅� ABP 婵冨害銆�

3. 鏁忔劅搴︼細1.0 pg/ml

娉ㄦ剰浜嬮爡(xi脿ng)锛�

1. 瀵�(sh铆)椹�(y脿n)鎿嶄綔涓繀闋堜娇鐢ㄤ竴娆℃€у惛闋紝閬垮厤浜ゅ弶姹℃煋銆�

2. 鍔犳ǎ锛氬姞妯ｆ檪(sh铆)锛岃鎺у埗鍔犳ǎ閫熷害锛岄伩鍏�**瀛旇垏鏈€鍚庝竴瀛旈枔鐨勬檪(sh铆)闁撻枔闅旈亷(gu貌)澶э紝鍚﹀墖灏囨渻(hu矛)灏�(d菐o)鑷翠笉鍚岀殑闋�(y霉)瀛佃偛鏅�(sh铆)闁擄紝寰炶€屽奖闊�

瀵�(sh铆)椹�(y脿n)鐨勬簴(zh菙n)纰烘€т互鍙婇噸寰�(f霉)鎬с€�

3. 瀛佃偛锛氬毚(y谩n)鏍兼寜鐓ц(shu艒)鏄庢浉(sh奴)涓婅(gu墨)瀹氱殑瀛佃偛鏅�(sh铆)闁撳拰婧害閫�(j矛n)琛屻€�

4. 鍙嶆噳(y墨ng)鏅�(sh铆)闁撶殑鎺у埗锛氬姞鍏ュ簳鐗╁悗璜�(q菒ng)瀹氭檪(sh铆)瑙€瀵熷弽鎳�(y墨ng)瀛旂殑椤忚壊璁婂寲锛屽鏋滈鑹茶純娣憋紝璜�(q菒ng)?zh铆)宕嗗嚬灏よ櫌K姝㈡恫绲傛鍙嶆噳(y墨ng)銆�

5. 寤鸿瀵�(sh铆)椹�(y脿n)鍓嶉爯(y霉)娓�(c猫)妯ｅ搧鍚噺锛屽妯ｅ搧婵冨害閬�(gu貌)楂橈紝鎳�(y墨ng)灏�(du矛)妯ｅ搧閫�(j矛n)琛岀█閲嬶紝瑷�(j矛)绠楃祼(ji茅)鏋滄檪(sh铆)涔樹互鐩告噳(y墨ng)鐨勭█閲嬪€嶆暩(sh霉)銆�

6. 寤鸿浣跨敤鏈│鍔戠洅鏅�(sh铆)鍏堝仛闋�(y霉)瀵�(sh铆)椹�(y脿n)锛堝嵆鍏堝仛妯�(bi膩o)婧�(zh菙n)鏇茬窔锛岃│鐢ㄥ咕鍊�(g猫)妯�(bi膩o)鏈級锛屽鏋滃皪(du矛)鏈│鍔戠洅鏈変换浣曠枒鍟�(w猫n)锛屽彲鍜屾墍璩�(g貌u)缍�(j墨ng)閵峰晢鑱�(li谩n)绯伙紝

濡傛灉鍥犻亱(y霉n)杓搁亷(gu貌)绋嬪皫(d菐o)鑷磋│鍔戠洅澶辨晥锛屽彲瑕佹眰瑾�(di脿o)鎻涳紝浣嗘涓嶆壙鎿�(d膩n)鐢�(ch菐n)鍝佹湰韬互澶栫殑浠讳綍鎼嶅け銆�

PKB Elisa Kit瀹夊叏鎬�

1锛� 閬垮厤浜洪珨鐩存帴鎺ヨЦ椤壊鍔慉銆丅鍜岀祩姝㈡恫銆備竴鏃︽帴瑙稿埌閫欎簺娑查珨锛岃珛(q菒ng)鐩″揩鐢ㄦ按娌栨礂銆�

2锛� 瀵�(sh铆)椹�(y脿n)涓笉瑕侀€�(j矛n)椋熴€佹娊鐓欐垨浣跨敤鍖栧鍝併€�

3锛� 涓嶈鐢ㄥ槾鍚稿彇瑭﹀姂鐩掗噷鐨勪换浣曟垚浠姐€�

淇濆瓨姊濅欢鍙婃湁鏁堟湡锛�

1.瑭﹀姂鐩掍繚瀛橈細2-8鈩�

2.鏈夋晥鏈燂細6鍊�(g猫)鏈�